Karenliu

The Liu Lab

Department of Craniofacial Biology

Kings College London

Home

Research

Publications

Protocols & Etc.

Lab Members

Contact Us

Links

Welcome to the Liu Lab!

We are interested in signal transduction in developmental biology.

In any intricate developmental process, the encoded genes are interpreted as myriad intercellular and intracellular interactions, all of which must occur at the right time and place. With this in mind, we are developing new methods to block or alter the activities of individual proteins in a drug-dependent manner. Our goal is to expand the repertoire of molecular tools available to developmental biologists. Rather than screen for small molecule-protein interactions we have chosen to use a directed approach to making chemically sensitive protein alleles, in which we use drug-dependent tags to regulate protein stability and localization.

The biological problem that we are interested in is the development of the neural crest and its derivatives, including the craniofacial skeleton. At present, we are developing chemical tools to study the roles of GSK-3 and Wnt signaling in the neural crest. We are using two model systems, the frog Xenopus laevis and the mouse. Xenopus embryos are abundant and live in an aquatic environment, allowing easy manipulation and drug accessibility; thus, we are using Xenopus to study early patterning and to rapidly test new tools. We then adapt these tools to mammalian systems. In the mouse, we are currently studying the development of the bony skull, using conventional and drug-dependent alleles of GSK-3β.

<wikionly>

Recent updates to the lab wiki

List of abbreviations:

N

This edit created a new page (also see list of new pages)

m

This is a minor edit

b

This edit was performed by a bot

(±123)

The page size changed by this number of bytes

4 May 2024

18:10

The Paper that Launched Microfluidics - Xi Ning‎ diffhist −86‎ Xning098 talk contribs (→‎Electrokinetic effect)

11:36

User:Jernej Turnsek‎ diffhist +50‎ Jernej Turnsek talk contribs

			09:20		User:Massih Forootan‎‎ 3 changes history +83‎ [Massih Forootan‎ (3×)]
					09:20 (cur | prev) +78‎ Massih Forootan talk contribs (→‎Link to Publications)
					09:18 (cur | prev) 0‎ Massih Forootan talk contribs (→‎Skills)
					09:18 (cur | prev) +5‎ Massih Forootan talk contribs (→‎Skills)

07:18

Move log Sarah L. Perry talk contribs moved page Microfluidic Gradient Generators - Greg Schneider to Microfluidic Gradient Generators ‎(Adding an Author)

00:15

Renhao Li Lab:Publications‎ diffhist +265‎ Renhao Li talk contribs (→‎2024)

3 May 2024

15:02

CHIP:Talks‎ diffhist 0‎ Gabor Balazsi talk contribs

15:01

CHIP:Data‎ diffhist −23‎ Gabor Balazsi talk contribs

			14:33		UA Biophysics:Protocols:Kanamycin‎‎ 5 changes history −421‎ [Elizabeth Suesca‎ (5×)]
					14:33 (cur | prev) +13‎ Elizabeth Suesca talk contribs
					14:21 (cur | prev) +16‎ Elizabeth Suesca talk contribs (→‎Materials)
					14:19 (cur | prev) −6‎ Elizabeth Suesca talk contribs
					14:14 (cur | prev) −50‎ Elizabeth Suesca talk contribs
					14:13 (cur | prev) −394‎ Elizabeth Suesca talk contribs

			14:33		UA Biophysics:Protocols:Ampicillin‎‎ 4 changes history −379‎ [Elizabeth Suesca‎ (4×)]
					14:33 (cur | prev) +15‎ Elizabeth Suesca talk contribs
					14:19 (cur | prev) −7‎ Elizabeth Suesca talk contribs
					14:11 (cur | prev) 0‎ Elizabeth Suesca talk contribs (→‎PROTOCOL)
					14:09 (cur | prev) −387‎ Elizabeth Suesca talk contribs

			14:31		UA Biophysics:Protocols:Erythromycin‎‎ 3 changes history −315‎ [Elizabeth Suesca‎ (3×)]
					14:31 (cur | prev) −7‎ Elizabeth Suesca talk contribs
					14:30 (cur | prev) −12‎ Elizabeth Suesca talk contribs (→‎Materials)
					14:18 (cur | prev) −296‎ Elizabeth Suesca talk contribs

			14:29		UA Biophysics:Protocols:Chloramphenicol‎‎ 2 changes history −427‎ [Elizabeth Suesca‎ (2×)]
					14:29 (cur | prev) +36‎ Elizabeth Suesca talk contribs
					14:16 (cur | prev) −463‎ Elizabeth Suesca talk contribs

			N    12:09		BioMicroCenter:Oligo Synthesis‎‎ 7 changes history +4,408‎ [Noelani Kamelamela‎ (7×)]
					12:09 (cur | prev) −14‎ Noelani Kamelamela talk contribs (→‎Dr Oligo 96)
					12:09 (cur | prev) −16‎ Noelani Kamelamela talk contribs (→‎STX-200)
					12:08 (cur | prev) −46‎ Noelani Kamelamela talk contribs (→‎Dr Oligo 96)
					12:08 (cur | prev) +35‎ Noelani Kamelamela talk contribs (→‎Dr Oligo 96)
					08:29 (cur | prev) +7‎ Noelani Kamelamela talk contribs (→‎Dr Oligo 96)
					08:27 (cur | prev) +59‎ Noelani Kamelamela talk contribs (→‎Dr Oligo 96)
			N		08:16 (cur | prev) +4,383‎ Noelani Kamelamela talk contribs (Created page with "{{BioMicroCenter}} ''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR> The BioMicro Center currently hosts two oligo synthesizers: one Dr Oligo 96 (Biolytic) and one Syntax STX-200 (DNAScript). <br><br> Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the...")

			12:06		BioMicroCenter:Covaris‎‎ 5 changes history +172‎ [Noelani Kamelamela‎ (5×)]
					12:06 (cur | prev) −5‎ Noelani Kamelamela talk contribs (→‎R230)
					09:55 (cur | prev) +2‎ Noelani Kamelamela talk contribs (→‎R230)
					09:54 (cur | prev) +1‎ Noelani Kamelamela talk contribs (→‎R230)
					09:52 (cur | prev) +4‎ Noelani Kamelamela talk contribs (→‎R230)
					08:41 (cur | prev) +170‎ Noelani Kamelamela talk contribs (→‎R230)

			09:52		(Upload log)‎ [Noelani Kamelamela‎ (6×)]
					09:52 Noelani Kamelamela talk contribs uploaded File:R230pic.jpg ‎
					08:42 Noelani Kamelamela talk contribs uploaded File:R230pic.jpeg ‎
					08:32 Noelani Kamelamela talk contribs uploaded File:Syntax.jpg ‎
					08:31 Noelani Kamelamela talk contribs uploaded File:Droligoprocess.jpg ‎
					08:29 Noelani Kamelamela talk contribs uploaded File:Chamber.jpg ‎
					08:28 Noelani Kamelamela talk contribs uploaded File:DrOligo.jpg ‎

2 May 2024

</wikionly>

<nonwikionly> This page was created using Open Wetware.</nonwikionly>